2. Anti-infection
  3. EBV CMV
  4. Maribavir

Maribavir  (Synonyms: 马立巴韦; 1263W94; BW1263W94; GW257406X)

目录号: HY-16305 纯度: 99.72%
COA 产品使用指南

摩尔计算器

Maribavir 是一种有效抑制野生型 pUL97 催化的组蛋白磷酸化，IC50 为 3 nM。Maribavir 可有效作用于 HCMV 和 Epstein-Barr 病毒 (EBV)。

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Maribavir Chemical Structure

Maribavir Chemical Structure

CAS No. : 176161-24-3

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规格 价格 是否有货 数量
1 mg ￥845
In-stock
5 mg ￥2013
In-stock
10 mg ￥3179
In-stock
25 mg ￥5313
In-stock
50 mg 现货 询价
100 mg 现货 询价
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Maribavir:

Maribavir 相关抗体

Top Publications Citing Use of Products

    Maribavir purchased from MCE. Usage Cited in: J Biol Chem. 2019 Apr 12;294(15):6188-6203.  [Abstract]

    Total cell lysates are subjected to CoIP and IVKA. Each IVKA reaction is supplemented by addition of either CDK7-cylin-H-MAT1 complex (200 ng), Rb-CTF (1 μg), CDK7 inhibitor LDC4297 (1 μM) or pUL97 inhibitor MBV (3 μM) as indicated.

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Maribavir is a potent inhibitor of histone phosphorylation catalyzed by wild-type pUL97 in vitro, with an IC50 of 3 nM. Maribavir has potent antiviral activity against HCMV and Epstein-Barr virus (EBV).

    IC50 & Target

    HCMV[1]
    体外研究
    (In Vitro)

    Maribavir is a potent inhibitor of the autophosporylation of the wild type and all the major Ganciclovir (GCV) resistant UL97 mutants analysed with a mean IC50 of 35 nM. The M460I mutation results in hypersensitivity to Maribavir with an IC50 of 4.8 nM. A Maribavir resistant mutant of UL97 (L397R) is functionally compromised as both a Ganciclovir kinase and a protein kinase (~ 10% of wild type levels). Enzyme kinetic experiments demonstrate that Maribavir is a competitive inhibitor of ATP with a Ki of 10 nM[1]. Maribavir (1263W94) inhibits viral replication in a dose-dependent manner, with IC50 of 0.12±0.01 μM as measured by a multicycle DNA hybridization assay. The pUL97 protein kinase is strongly inhibited by Maribavir, with 50% inhibition occurring at 3 nM[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Maribavir 相关抗体:
    Clinical Trial
    分子量

    376.24

    Formula

    C15H19Cl2N3O4
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    马立巴韦
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 200 mg/mL (531.58 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.6579 mL 13.2894 mL 26.5788 mL
    5 mM 0.5316 mL 2.6579 mL 5.3158 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 2.87 mg/mL (7.63 mM); 澄清溶液

    • 方案 二

      请依序添加每种溶剂： 5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.87 mg/mL (7.63 mM); 澄清溶液

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.72%

    COA (200 KB) LCMS (87 KB) 元素分析报告 (216 KB)

    产品使用指南 (1538 KB)
    参考文献
    Kinase Assay
    [1]

    Enzyme kinetic analysis is performed on the purified wild type and mutant UL97 protein species using increasing concentrations of ATP (2 μM to 20 μM). The amount of incorporated radiolabelled phosphate is plotted against the concentration of ATP in a Lineweaver Burke plot to determine the Km for ATP for each UL97 species. The effect of Maribavir upon the rate of radiolabelled phosphate incorporation by wild type or mutant UL97 is determined by protein kinase assays at a fixed concentration of Maribavir (0.5 μM) as above, or with increasing concentrations of Maribavir (0.01 μM to 5.0 μM) to determine the IC50 of Maribavir for each UL97 species. In order to determine the nature of the inhibition mediated by Maribavir, plots of 1/v vs 1/ATP with increasing concentrations of Maribavir are constructed. Competitive inhibition is evident if the family of lines cconverged on the y-axis at 1/Vmax. The change in slope caused by the addition of Maribavir is used to calculate the Ki[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [2]

    For these studies MRC-5 cells are seeded in 24-well plates at ~5×104 cells/well and grown for 3 days in MEM 8-1-1 to confluence (~1.1×105 cells/well). The cells are infected with AD169 in MEM 2-1-1 at an MOI ranging from 1 to 3 and incubated at 37°C for 90 min to allow viral adsorption. The unadsorbed virus is removed and replaced with 1 mL of MEM 2-1-1. To test the effect of compounds on viral DNA synthesis or maturation, Maribavir, BDCRB, or GCV is added to the medium at the concentrations indicated for each experiment[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Maribavir 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6579 mL 13.2894 mL 26.5788 mL 66.4470 mL
    5 mM 0.5316 mL 2.6579 mL 5.3158 mL 13.2894 mL
    10 mM 0.2658 mL 1.3289 mL 2.6579 mL 6.6447 mL
    15 mM 0.1772 mL 0.8860 mL 1.7719 mL 4.4298 mL
    20 mM 0.1329 mL 0.6645 mL 1.3289 mL 3.3223 mL
    25 mM 0.1063 mL 0.5316 mL 1.0632 mL 2.6579 mL
    30 mM 0.0886 mL 0.4430 mL 0.8860 mL 2.2149 mL
    40 mM 0.0664 mL 0.3322 mL 0.6645 mL 1.6612 mL
    50 mM 0.0532 mL 0.2658 mL 0.5316 mL 1.3289 mL
    60 mM 0.0443 mL 0.2215 mL 0.4430 mL 1.1074 mL
    80 mM 0.0332 mL 0.1661 mL 0.3322 mL 0.8306 mL
    100 mM 0.0266 mL 0.1329 mL 0.2658 mL 0.6645 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Maribavir176161-24-31263W94 BW1263W94 GW257406X马立巴韦EBVCMVEpstein-Barr VirusCytomegalovirusInhibitorinhibitorinhibit

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